T-DNA:
LB microhomology/ LB truncation/ Precise RB
Illegitimate, simple, direct or inverted repeats, single copy
Target small deletion/ duplication/ recombination
Integration pattern: stable/ transient
Biolistic:
Co-bombardment/ co-segregation/ complex
Silencing:
Gene dosage: single copy: homozygous silencing
High expression/ strong mRNA production, Multi-copy transgene
Heterochromatin/ repeat arrays interaction
Post-transcriptional silencing of single copy/ strong promoter
Endogenous/ environmental promoter methylation
Direct gene transfer
Host and plasmid rearrangement, multicopy, interspersed insertion locus
Mechanism: wound response, repair, ligation, DSB receptive
Illegitimate Recombination:
Non-homologous/ eukaryotic/ random integration
Translocation, exon-shuffling/ gene amplification/ filler DNA
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